Amos Frost was born in Pennsylvania, the son of William and Nancy Richey Frost. He moved to Indiana as a young man. Soon after his arrival in Vernon, Jennings County, Indiana, he began to publish a newspaper, called "The Experiment." In 1844 he was elected Recorder of Jennings County. He then attended the UofL Department of Medicine, graduating in 1850. Afterward he practiced in Vernon and Seymour, Jackson County. In 1873 he moved to Thomasville, Georgia due to ill health. In 1878, he returned to Vernon to be near family, and died there two years later.